FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6098503
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252890.41467553594710044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117050.19193234798769468No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109060.1788307720763604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA301800.066.2411351
GGTATCA134850.062.7111781
TATCAAC424150.047.0994452
ATCAACG427050.046.668293
CAACGCA427850.046.593395
AACGCAG429600.046.5661286
TCAACGC436350.045.8230554
ACGCAGA447700.044.5902527
CGCAGAG450850.044.3299678
GCAGAGT490900.040.7133149
AGAGTAC472300.033.15267610-11
CAGAGTA489050.028.91326710-11
AGTACGG392650.028.86996512-13
TACGGGT94000.026.73027214-15
AGTACTT159800.026.6572812-13
GAGTACT145950.026.31709112-13
TACGGGC89750.025.67685314-15
TACGGGG107700.025.67685114-15
GAGTACG403700.025.5190112-13
GTACGGG402900.024.65776414-15
Produced by FastQC (version 0.11.5)