FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6098503
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN63430.10400913142126847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA240200.063.6317371
GGTATCA112700.055.324821
CAACGCA328000.045.992465
AACGCAG331400.045.695886
ACGCAGA331900.045.4505167
TATCAAC337050.045.2396352
ATCAACG336000.045.0084343
CGCAGAG335650.044.9777838
TCAACGC339650.044.524764
GCAGAGT370200.040.7690669
AGAGTAC354100.032.9588310-11
AGTACGG398850.028.9991712-13
CAGAGTA371900.028.35114510-11
TACGGGC88700.026.78854214-15
TACGGGT94950.026.4972814-15
TACGGGG106650.026.15579214-15
GAGTACG409000.025.63330312-13
GTACGGG411150.024.6287714-15
TACGGGA156600.022.54160714-15
ACGGGTA30250.019.66730116-17
Produced by FastQC (version 0.11.5)