FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5398986
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT423920.7851844772333175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188740.3495841626557283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188470.3490840687492059No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA309800.070.3578641
GGTATCA128400.067.754911
TATCAAC436150.050.054122
ATCAACG438050.049.6471063
CAACGCA442200.049.2747845
TCAACGC443000.049.2399064
AACGCAG451750.048.2694446
ACGCAGA477900.045.552777
CGCAGAG493650.044.0981758
GCAGAGT513900.042.418319
AGAGTAC495400.034.36597410-11
CAGAGTA504300.030.24274310-11
AGTACGG355400.029.43921312-13
AGTACTT223650.029.2085912-13
GAGTACT221350.026.53269812-13
TACGGGT66500.025.846714-15
GAGTACG363150.025.82514812-13
GTACTTT233950.025.16755714-15
TACGGGG123150.024.46098714-15
GTACGGG374450.024.31701714-15
Produced by FastQC (version 0.11.5)