FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4282331
Sequences flagged as poor quality0
Sequence length150
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156300.3649881337990922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87600.20456148765707274No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA81400.19008339149869544No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC74580.17415748572448042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72150.1684830061011164No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT68770.16059010851800107No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC68530.16002966608606387No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT56360.1316105644332491No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA54250.12668334138580134No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC47580.11110771213154705No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC47400.11068738030759416No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC43420.10139337664463582No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42950.10029584354875884No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA205950.078.687251
GGTATCA104500.072.453281
CAACGCA318550.051.347835
TATCAAC318650.051.3299262
ACGCAGA326850.049.977837
CGCAGAG325500.049.8754658
AACGCAG330950.049.488626
GCAGAGT328400.049.3692679
ATCAACG337900.048.449993
TCAACGC344750.047.466434
TCTCTGC21100.034.8023958
ATTTAGG40550.033.3935851
TTCTCTG22800.033.1547557
TTTAGGG41400.031.6480712
TAAGACT25850.029.5213839
CTATTGA47350.028.584419
CAGAGTA326350.028.3031279
ATTCTCT27650.027.0784956
TACATGC29800.026.5737342
TATTCTC28950.026.3601995
Produced by FastQC (version 0.11.5)