FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5463830
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA281750.5156639207295981No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG262740.480871476601578No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT203020.37157085780487314No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT202010.3697223376276348No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT184090.3369248311166343No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG140940.2579509245346213No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA138120.2527897097823322No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC130510.23886175082314054No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT127150.23271221835232794No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125700.23005840225629273No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT123500.2260319226623083No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC117950.21587421277748392No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT112380.20567989853271423No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA111450.20397779579525718No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG104920.19202647227311245No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT101760.186242983401753No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG95780.17529827977810436No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA91600.16764796854953393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91240.1669890900705183No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA88520.16201089711795572No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT84090.15390303139006886No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG81250.14870521227783443No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG80830.14793652071898283No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG79570.1456304460424281No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT78570.14380022804516246No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC78080.1429034212265023No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTT76040.13916977651208037No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA69530.12725505734988096No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA69160.12657787669089265No Hit
AGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATG68710.1257542785921231No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT68350.12509540011310746No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT67400.1233566930157051No Hit
GCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATA66070.12092250307934177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65180.11929360906177534No Hit
ACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGG63770.11671300168563077No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTT63500.11621884282636906No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC62460.11431541610921277No Hit
AGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCAT60980.1116066934732596No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA60960.11157008911331429No Hit
TCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTA60950.11155178693334164No Hit
ATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAAT60630.11096611717421662No Hit
ATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACC59850.10953854713634942No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT58870.10774493349902908No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGG58350.10679322014045094No Hit
AAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCA58200.10651868744086107No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC57230.1047433759835134No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT57180.10465186508365013No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT55700.10194314244769695No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT55660.10186993372780632No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT55380.10135747268857193No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG54880.1004423636899391No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAG2800.080.7262341
ATACATG17250.072.413461
GGTATCA66950.070.543831
GTATCAA171600.050.885951
ATCGTAA2450.046.1292761
ACGCAAT2500.045.2066881
TCCAGTA10250.044.1036832
ATCAACG238000.042.612063
TCAACGC243300.041.7330864
CAACGCA246550.041.158845
ACTTGTA3650.040.741431
AACGCAG262800.038.637166
ACGGTAT1551.2187229E-1038.3757971
ATATACG2500.038.068791
GTACACT11850.037.145866
ACTCTAT5000.036.8791431
ACGCTAT851.714287E-434.9896971
ATACGAC2750.034.6076743
ACCCGTG1754.474714E-1033.989991
ACCTTAG102200.033.989991
Produced by FastQC (version 0.11.5)