FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7363593
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92310.1253599974903556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA206200.050.826241
GGTATCA116500.048.2476961
TCAACGC256050.041.4199034
ATCAACG259300.040.8778153
CAACGCA261700.040.48025
AACGCAG273700.038.7708636
TATCAAC302350.035.35232
ACGCAGA319300.033.2525337
CGCAGAG327550.032.4331638
GCAGAGT354050.030.0723929
AGAGTAC339800.025.18917810-11
ATACGAC4550.024.8384633
GTACATG377850.023.9903321
TACATGG381550.023.4619752
ACATGGG369350.023.3996853
CAGAGTA353750.023.29628610-11
GAGTACT100450.021.87961412-13
GAGTACA289200.020.95805712-13
AGTACAT307950.020.84089512-13
CATGGGA207600.019.4547184
Produced by FastQC (version 0.11.5)