FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6083969
Sequences flagged as poor quality0
Sequence length150
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138680.22794330477357788No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC107680.17698972496408183No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG98280.16153928463475076No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA90070.1480448043045584No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC84290.13854442716588464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82950.13634191758702255No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA81980.13474756363814477No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA78230.12858382414506056No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT75120.12347202952546273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70730.11625634515889216No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC70200.11538520331053626No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT68580.1127224678495239No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA67680.11124317037118367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62750.069.317931
GTATCAA122300.065.2239151
CAACGCA143150.056.026635
TCAACGC141050.055.8905144
ATCAACG141400.055.5485153
AACGCAG147650.054.17286
ACGCAGA153000.052.466747
CGCAGAG155600.051.3128558
TATCAAC167100.047.6525882
GCAGAGT183600.042.708029
GACCGTT8700.042.2038047
GTACTGG55550.030.7429031
GGACCGT16800.030.426346
GTATAGG69250.030.3518961
GCGTACA25200.029.7125972
GGTTCAC41350.028.3800246
AGCGTAC27650.027.7145121
GAGTACG126100.026.6229291
TAGGGGT27050.026.6152064
TACACGG27350.026.323485
Produced by FastQC (version 0.11.5)