FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004922658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2953948
Sequences flagged as poor quality0
Sequence length125
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT62500.21158124652160432No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGC49660.1681139952362059No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43730.14803916656623609Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT43550.14742981257625387Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGT43200.1462449575957329No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT43170.14614339859740252No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTA35850.12136300300479223No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGC35310.11953494103484558No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGGGGTG32880.11130866217008559No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACA32790.1110039851750945No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)