FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15626215
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA23000.033.4501959
CCCGTAT43900.030.1091081
GTATCGA42000.029.9645734
CGACCGT18000.029.0561331
ACCGTTG18700.026.9200443
GAACGTA15750.026.8361954
AACGTAT15850.026.6668835
CGACCTA5800.026.201944
CGTATCG49750.025.6785623
ACGTATT16550.025.2520246
CCGTATC56800.023.1602342
CTATAAC33550.022.958321
GACGCTA59750.022.4141734
TCGAACG21500.021.87082740-41
GTGCGCG37050.021.662578
TTGAACG48050.021.2498867
CCGTCAT42750.021.0205121
ACGCTAC64300.020.9019575
ACGTGCG39000.020.3358976
AACACGT40800.019.2059273
Produced by FastQC (version 0.11.5)