FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15626215
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA24250.035.0629659
CGACCGT19700.030.8608571
GTATCGA41500.030.3312174
CCCGTAT41950.030.230381
GAACGTA13050.026.9347694
CGACCTA8100.026.3887964
ACCGTTG20800.026.2619253
CGTATCG49300.026.0141073
AACGTAT13800.024.438325
GTGCGCG39050.023.3554848
TTGAACG47750.022.482537
ACGTATT14950.022.241656
ACGTGCG42000.022.167516
CTATAAC31750.021.8410431
GACGCTA57600.020.8636424
CCGTATC63400.020.8276672
AACACGT44850.020.3343753
ACGCTAC60150.020.1370855
TCGAACG24400.019.95484540-41
GAACGCT53450.019.6404729
Produced by FastQC (version 0.11.5)