FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19048158
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA12400.029.4961914
TTGAACG50400.024.787617
CCCGTAT35400.023.3468381
GTATCGA28900.021.2025814
CGTATCG29900.020.8111933
GAACGCT60800.020.07879
TCGAACG29200.019.9275140-41
GAACGTA18400.019.1034054
CGACCGT15900.016.7291161
ATTGAAC84600.016.6761426
TGCGCGA24000.016.6254759
AACGTAT19550.016.2788945
ACGGTAA36850.015.79017544-45
CTCAGAT134750.015.7212891
GACGCTA45950.015.5061034
TACGCAC23150.014.978839
TACCGTA35300.013.9947359
ACGTATT23600.013.8882196
AGTCGAA43100.013.88656238-39
CGAACGG42350.013.85202740-41
Produced by FastQC (version 0.11.5)