FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16321621
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT489210.2997312583106788TruSeq Adapter, Index 19 (97% over 40bp)
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT248240.15209273637710372No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA202460.12404405175196753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT14000.039.447041
TCGTATG88250.038.51655244-45
ACCGTTG13600.035.6209343
TGAAACG99350.034.23138434-35
CAGATTG59350.033.1301653
GCCGTCT103050.032.13117650-51
GATCTCG89900.030.964140-41
TTGAACG65950.030.461487
GTCACGT113550.030.27302428-29
TATGCCG91200.030.02928448-49
CGTATGC92150.029.6944346-47
CTCGTAT90900.029.50207344-45
ACGATCT94550.029.41295438-39
ATCTCGT88700.028.92113142-43
CCGGTAG87600.028.3694461
GTATCGA47350.027.6842734
ATTGAAC75250.027.6437536
CACGTGA124750.027.55470330-31
CGTCTTC124200.026.98261352-53
CCGTCAT43700.025.819711
Produced by FastQC (version 0.11.5)