FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23499309
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTGGGGCTTGTCC246260.10479457076801706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTAG80950.032.1509135
TGCGCGA48750.030.4929439
TGACCTA86250.029.5144924
ACCTAGC94900.027.6250156
AACACGT77650.021.0400433
ACGTGCG78150.020.6623446
GTGCGCG78350.019.3366538
TAGCAGT135850.019.1580169
TAACACG88250.018.1899552
GTATCGA27300.017.9186064
TCTGACC160550.017.245982
CTCAGAT197850.016.701891
GATTTAA131350.016.379395
CGTATCG30100.016.2517573
CTAGCAG166050.015.9597078
CGCGAGT48650.015.66829810-11
CTCTGAC200450.015.3467041
ACACGTG106950.014.9206624
CCCGTAT31900.014.9048011
GCTTAAT87500.014.1280481
Produced by FastQC (version 0.11.5)