FastQCFastQC Report
Sun 5 May 2024
EGAF00004922751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32374682
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT745280.2302045777623391Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG544240.16810667051494127Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCGT365960.11303894815090385Illumina Single End PCR Primer 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)