FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9435128
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT13900.014.3784271
CGACCTA6501.9826984E-1012.4221544
CTCGCGT8155.456968E-1211.6774831
CGATCGG13300.09.9992599
CCCGGAT39400.09.662081
CGTATCG12051.8189894E-129.459883
GATCGGA51900.09.33644894-95
TACGCAC9206.1263563E-99.29278859
CTCGGAT29850.09.2461411
CGTATCC10456.1299943E-109.0902343
ATCGAAG28350.09.04694756
AGATCGG64600.09.04526894-95
GTATCGA11655.638867E-118.9692924
CGCCATT24950.08.9640481
TACACGC18550.08.9615992
TCGATCG14350.08.936628
TCACGTA25050.08.9115147
CGCGAAT5901.4427504E-48.8719191
TTCGCGC7552.963472E-68.8072946
TATGCCG40150.08.754023
Produced by FastQC (version 0.11.5)