FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9435128
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA7900.013.225354
CTCCGAT21150.011.0016581
CTCGGTT15250.010.5872091
TTCGCGC8106.8030204E-1010.5538437
CTCGACT22050.010.552611
TCCGATT16800.010.175842
TACGCAC11250.010.1316889
CCCCGTT18700.09.3957741
GATCGGA49500.09.35337694-95
CTCGGGT38400.09.27475551
CCCGGAT40250.09.2024011
CCGATTA10951.546141E-109.1078683
TCGGGTC40350.09.0620042
CGCCAAC24500.08.9158791
CTCGAAT21250.08.7152151
CGATTAG16950.08.6856764
AGATCGG65750.08.5583894-95
CTCCCGT37200.08.5527211
CTCAGAT64000.08.5327751
GTTCGAT19550.08.5014641
Produced by FastQC (version 0.11.5)