FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13605029
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG1924741.414726863132743TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAAACTCGTATG1750281.2864948689194267TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAAAATCGTATG172020.12643853974879438TruSeq Adapter, Index 15 (97% over 39bp)
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT160930.118287142203078No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTATG147330.10829083863033294TruSeq Adapter, Index 15 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG583350.084.360472
ATCGGAA587750.084.054651
AGAGCAC634100.078.275287
GAGCACA638700.077.570268
AGCACAC641700.077.207619
CGGAAGA639450.076.9668053
AAGAGCA654800.074.9884646
GAAGAGC774350.063.7117045
GGAAGAG821850.060.058294
TCGTATG523550.044.42889444-45
ACACGTC558250.044.20868312-13
ACGTCTG559900.043.8409814-15
AAACTCG242700.043.8365940-41
CTCGTAT500000.043.80188442-43
AACTCGT241800.043.5969940-41
CACGTCT565450.043.49458312-13
GCACACG582350.042.6033410-11
CGTATGC542600.042.4531944-45
GTCACAT557100.042.3220728-29
CACACGT580550.042.28550310-11
Produced by FastQC (version 0.11.5)