FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13605029
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT766640.5634975125742107Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCGT316950.23296532480746643Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGG252530.185615186854802Illumina Single End PCR Primer 1 (97% over 49bp)
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT149670.11001079086270231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT411300.084.748947
TCGGAAG518250.082.990362
ATCGGAA528750.082.795911
AAGAGCG504950.082.4911356
AGCGTCG456150.077.216519
GAGCGTC464450.074.866498
CGGAAGA578500.074.248513
AGAGCGG149100.074.1267247
AGCGGCG176650.063.371519
GAGCGGC176950.062.003688
GAAGAGC690550.060.703945
ATCGGGA86850.057.3078351
CGGGAGA104350.045.649813
GGAAGAG957450.045.4171834
GTAGATC491700.043.82575630-31
CGTATCA323700.043.6175448-49
GATCTCG491550.043.2793432-33
GCCGTAT329100.043.27692446-47
GCGTCGT357300.043.20145810-11
TGGTCGC342350.043.04804240-41
Produced by FastQC (version 0.11.5)