FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19096418
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGTGTTGTGTATGGTTATCATTTGTTTTGAGGTTAGTTTGATTAGT201160.10533912695040505No Hit
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATT193130.10113414987041025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT82650.046.306597
CGACCGT19900.042.5324821
TCGGAAG106250.042.188242
AAGAGCG120250.036.605416
ACCGTTG21950.036.5573233
TGCGCGA33200.034.61219
TTGAACG50900.031.5318587
ATCGGAA146650.031.51651
AGAGCGG40850.029.0601987
AGCGTCG134150.029.0251059
GTAGATC87300.027.39047430-31
GTGCGCG42550.027.3411758
GATCTCG84750.026.8415332-33
GACGCTA65950.026.5663244
ACGCTAC65600.026.418625
GCCGTAT62800.026.05974646-47
GCGTCGT71200.025.50944110-11
GTATCGA54600.025.3927734
TCGGTGG81750.025.03955536-37
CTATAAC31750.024.8609731
Produced by FastQC (version 0.11.5)