FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8677751
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA24800.032.7463849
GTGCGCG32700.025.1256528
CGACCGT7650.023.6743351
ACCGTTG7650.022.3779813
GACGCTA23350.021.1800174
ACGCTAC23500.021.0448255
CGCGAGT24850.017.8691610-11
AACGTAT6000.017.4361785
CGCTACT27300.017.3990826
AACACGT57350.017.246853
CGATAAC16450.016.5144671
GTATCGA15050.016.4303464
ACGTGCG60400.016.3574436
GAACGTA5600.016.1341254
TATGCCG23900.016.1163623
TAACACG62300.015.87651352
CGTATGC24100.015.8208041
CGAGTCG28100.015.71793912-13
ACGTATT6350.015.7084976
ACACGTG66450.015.0996664
Produced by FastQC (version 0.11.5)