FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8677751
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA26650.034.2105149
GTGCGCG34750.026.9194988
CGACCGT6500.025.6046471
GAACGTA5250.024.419264
ACCGTTG7200.021.1030653
AACACGT55800.019.4862963
GACGCTA22950.019.447924
ACGTGCG57800.019.3061456
AACGTAT6100.018.68145
ACGCTAC24200.018.6395855
CGCGAGT27700.017.31388910-11
TAACACG63500.017.1996542
GTATCGA16750.016.7249664
ACACGTG66700.016.0171324
CGCTACT28150.015.8564156
ACGTATT7250.015.7191416
CGAGTCG32100.015.088412-13
CACGTGC72800.015.0011475
CGTGCGC75650.014.4996867
CGATAAC17200.014.3760311
Produced by FastQC (version 0.11.5)