FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11298305
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT182170.161236574866761No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC138670.12273522444295848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA49150.038.6438649
GTGCGCG59450.032.1882448
TTGAACG25850.028.83927
CGACCGT9900.027.8998221
ACCGTTG9450.026.1488193
GCGAACG7950.022.0993087
AACACGT98600.021.8806133
ACGTGCG99700.021.7653246
GTATCGA16750.020.994114
ATTGAAC40600.020.4670896
CGCGAGT50000.020.37048510-11
TAACACG109450.019.7115422
CGAGTCG53600.018.91372912-13
CTCAGAT68550.018.5486561
ACACGTG116750.018.3569364
GAACGCT43750.018.233729
TCGAACG20200.017.98812340-41
CCCGTAT20950.017.957741
CACGTGC124950.017.2283025
CGTGCGC126650.017.208847
Produced by FastQC (version 0.11.5)