FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13713359
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA23950.021.020549
CGACCTA8750.020.082524
GTATCGA18900.016.8359454
TAACACG48300.014.3558932
CCCGTAT23750.014.2123551
AACACGT52700.013.6078913
ACGTGCG50850.013.44928556
ATCGAAG45550.013.1374126
TACGCAC14150.013.0903639
GACCTAG30100.012.9379045
CGTATCG23000.012.8023113
CGACCGT9600.012.3805531
GTGCGCG41450.012.3747038
TGACCTA32300.012.3509764
CGCGGAT16400.012.1752181
CTCAGAT109300.011.7874651
CGCCAAC34800.011.7487181
CAATCGG18150.011.2516544
CTAACAC70300.011.0907221
CGCGAGT26250.010.8558910-11
Produced by FastQC (version 0.11.5)