FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18531397
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT259290.1399192948054591No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA236990.12788566344998167No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC199080.10742849014567007No Hit
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATT195750.10563153981321537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA56550.047.35849
GTGCGCG67350.040.539728
TTGAACG59500.037.827877
GTATCGA44100.032.324174
CGACCGT15900.031.7429921
CAGATTG80250.029.2500513
GAACGCT77250.029.136039
CTCAGAT151800.028.7318021
CCCGTAT55750.027.5011041
ACCGTTG16850.027.0717333
ACGTGCG120950.026.8534896
ATTGAAC88250.026.8495066
CTTGGAT111750.025.9056911
AACACGT125450.025.8698983
CGTATCG58150.025.4129683
CTATAAC32550.024.8677831
TCAGATT156600.024.6078572
TCGAACG45550.024.2958340-41
AACGCTG45750.024.18328710-11
CCGTATC63400.023.3835282
Produced by FastQC (version 0.11.5)