FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18531397
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT237910.12838211819648568No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA229340.12375753430785602No Hit
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATT214600.11580346586930279No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC210170.11341292833994114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA54850.044.2485549
TTGAACG59050.038.287827
GTGCGCG65450.037.3015638
CGACCGT14800.031.7588061
GTATCGA46250.031.6262744
CAGATTG79600.030.2485073
GAACGCT78500.029.465529
ATTGAAC86600.028.3012316
CTCAGAT153400.027.91721
CCCGTAT54750.027.7495881
CTTGGAT114700.025.9120391
TCGAACG44150.025.50104940-41
ACGTGCG117800.025.1601286
ACCGTTG15900.025.0894553
CGAACGG45250.025.03860940-41
AACGCTG46100.024.83033810-11
TCAGATT155350.024.3932152
AACACGT122900.024.1511153
GTCGAAC46950.023.98193638-39
CGTATCG62850.023.0464293
Produced by FastQC (version 0.11.5)