FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12587919
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT235740.18727479895604668No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG136430.10838169517932232No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC130570.10372643802363203No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC130390.10358344377652891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT17900.030.6076681
ACCGTTG17850.029.0049783
CGCGTAA2155.638867E-1126.5906051
CGCGTAT1309.802581E-625.6531141
GTATCGA34200.025.55474
ATTGAAC45550.024.505226
TTGAACG45650.024.0342927
CAGATTG47150.023.4724543
CCCGTAT41050.022.5151061
CCGGTAG114950.021.4687231
TGCGCGA16200.021.1070549
CAGACGT23050.020.04871
CGGTAGC121400.020.0421542
TCGAACG25300.019.99347940-41
GTCGGGA30250.019.8440651
CGACCTA3605.456968E-1219.7910064
AACGCTG26300.019.68252810-11
CCGTCAT34500.019.3327831
CTATAAC19300.018.7603821
CTCAGAT79750.018.4592151
Produced by FastQC (version 0.11.5)