FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12587919
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT220220.17494551720582252No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC137160.10896161629257385No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG126530.10051701158865099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA2400.029.7111871
CGACCGT19750.027.9209961
GTATCGA37150.027.7411824
ACCGTTG20650.026.2184563
GAACGTA12900.026.1392254
AACGTAT13250.025.8067785
TTGAACG43600.023.965297
CCCGTAT44300.023.9299141
CAGATTG45000.023.7461033
CGTATCG50600.022.1505153
ACGTATT15300.022.0393926
ATTGAAC47850.021.5382546
TGCGCGA17000.021.51289
CCGGTAG109450.021.325821
CCGTCAT33550.020.403751
GACCGTT27850.020.1223722
CGGTAGC115850.019.9233592
TCGAACG25850.018.92621440-41
TCGAAGG71300.018.5183287
AACGCTG26450.018.49552510-11
Produced by FastQC (version 0.11.5)