FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13586347
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT406570.29924894454705153No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC250040.18403769607827622No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT203630.14987840366509114TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT12550.042.509411
ACCGTTG13750.037.653073
TTGAACG38950.029.8755597
CCGGTAG137400.029.5368141
TCGTATG38000.029.18748744-45
TGCGCGA44650.028.7186859
CTCGTAT39650.027.4337844-45
TATGCCG40000.027.2527148-49
CGGTAGC149250.027.0317762
GAACGTA15250.026.7858354
CGTATGC41250.026.54260446-47
CAGATTG45150.026.4053863
CCGATCT41200.025.53681238-39
TAGCGGC159100.025.1074375
AACGTAT16600.024.607475
GTGCGCG52400.024.4711698
GTATCGA29050.024.1987084
ATTGAAC51900.023.611796
AGCGGCC168550.023.4743086
GTCCCGA47400.023.24897636-37
Produced by FastQC (version 0.11.5)