FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004922777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13586347
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT332130.24445864661045386No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC237990.17516849819896402No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG207140.15246187956188664Illumina Single End PCR Primer 1 (100% over 50bp)
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC139220.10247051690936498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)