FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14280527
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA148770.10417682764788723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGG2850.043.375311
CAGATTG31500.035.2806933
GTCGGGA16850.034.7071761
CGCGTAA1004.9244125E-528.5276091
CTATAAC19650.028.309841
GTATCGA51300.026.7554534
CCGTCAT37500.026.3721891
GAACGTA17450.026.1280864
ACGTATT17450.026.1280866
AACGTAT17600.025.9054035
CGACCGT22450.025.2025621
TTAAGGC61000.025.148067
AGGAACG21100.024.9845947
CCCGTAT56550.024.6348021
ACCGTTG22850.023.486813
CGGGAAC45900.023.4104521
AACGCTG22950.023.17752810-11
CTTGGAT82750.022.4083921
GACGCTA61800.022.286474
ACTTAGG36350.021.9501086
Produced by FastQC (version 0.11.5)