FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11857413
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT225240.1899571179649389No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC155690.13130182781016397No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC149520.1260983319042695No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG144230.12163698776453177No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT125670.10598433233286214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT14800.039.886941
ACCGTTG14700.036.8474273
CGCGTAA3450.034.497821
TGCGCGA36100.033.278929
GTGCGCG43250.028.5458588
GAACGTA6850.028.438914
AACGTAT7050.026.9581785
TTGAACG33350.024.2052157
ACGTATT8250.024.174156
GACCGTT22950.024.0156842
GCGTAAC5300.022.4121652
AGACGTG23450.022.287922
CAGATTG38850.022.1364123
ATTGAAC39750.021.6220726
CTATAAC18150.020.9837991
GTATCGA29250.020.95484
CGACTAT2509.267751E-920.9470771
CAGACGT26500.020.659641
CCGTCAT24000.020.6296981
AACGCTG20050.019.89393810-11
Produced by FastQC (version 0.11.5)