FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11857413
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT196190.16545767613896892No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC165680.13972693706460254No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC147840.12468149671433389No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG129650.10934088236616199No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT120160.10133745025158523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA4200.046.3476371
CGACCGT15550.036.0283741
ACCGTTG14900.035.3768773
TGCGCGA35850.030.7356289
GTATCGA30700.028.1524264
GCGTAAC7050.027.6153182
GTGCGCG42000.026.5746368
CGCGTAT1000.001659005223.7390331
CAGATTG37050.023.4555553
CCCGTAT39450.022.6257951
CGACTAT2755.2750693E-1122.4441781
GAACGTA6200.022.2120744
TTGAACG35800.022.1555357
AACGTAT6700.021.9733945
GACCGTT24200.021.5840342
ATTGAAC39000.021.4336836
CTCAGAT66950.020.494641
GACGCTA41050.019.78184
CCGTCAT26550.019.6707631
AACGCTG20300.019.53714810-11
Produced by FastQC (version 0.11.5)