FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8500671
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC213040.25061551023442735No Hit
CTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC164390.19338473398158804No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT144200.16963366774222882No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT122330.14390628692723198No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT103560.12182567705537597No Hit
TGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTA91390.1075091601592392No Hit
GCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGA88030.10355653100796394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG13150.028.35163722-23
GTATGGC32150.026.2950062
TGGCGAC35800.026.165941
TGCGCGA53050.024.6196339
TATGCCG102850.024.6125833
CGTATGC103350.024.3387551
TATGGCC37350.023.397073
ATGCCGT112900.022.4637154
GCCGTCT118000.021.4123276
GGCGACC45950.021.1886162
GTATGCC121600.020.895582
ATATCGC18400.020.64935120-21
AGTATGG40850.020.6031841
GATATCG18750.020.26389720-21
TGCCGTC125150.020.189015
ATGCATC39650.020.1233548
GATGCAT40600.019.1845687
TAAGCAT98700.019.1513848
CGTCTTC132900.018.9759628
GTGCGCG71400.018.8909768
Produced by FastQC (version 0.11.5)