FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14589020
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA29950.019.8211529
TATGCCG46550.017.9796243
CGTATGC46300.017.9116741
GTATCGA20450.014.8824464
CCCGTAT26750.014.6102611
TAACACG47200.014.5080192
CGCGATT5451.5279511E-1013.9923741
CGATAAC17750.013.6942971
ACGTGCG50200.013.6251086
GTGCGCG48000.013.554868
AACACGT52850.013.4968973
CGACCTA6401.0913936E-1113.3745814
CGTATCG23650.012.86875253
GAACGTA9750.012.6810844
CGCGTAT3408.0695274E-512.6162851
ATGCCGT72400.011.8885164
ATAACGT20050.011.8588693
CGCGAGT27250.011.67680510-11
CGCGGGT37800.011.600161
TTGAACG30100.011.5178677
Produced by FastQC (version 0.11.5)