FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6110230
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA2000.037.996754
TGCGCGA21350.025.1383659
CGACCGT6050.024.3826521
ACCGTTG5650.021.8565373
CGCTAGG2205.5428245E-821.629771
GTGCGCG27600.020.6504088
GTATCGA12300.020.0795824
TGGCGAC21850.019.1648671
ACGTATT5550.017.9714376
GAACGTA5050.017.869764
AACGTAT4900.017.4474895
CCGTCAT14950.017.1880741
CCGACAT15800.017.1669221
CGCGTAG2002.7251398E-416.6549241
TACTCTA14550.016.6480629
CTATAAC7650.015.5508171
TCGAAGG34500.015.4189717
TTGAACG14500.015.3952357
ATCGAAG35450.015.1397496
CAGACGT16000.014.8704671
Produced by FastQC (version 0.11.5)