FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13394325
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT169570.12659839148296015No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC163720.12223087016329677No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC134060.10008716378018302TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA56500.028.8299249
GTGCGCG75550.022.06338
GAACGTA10850.021.0115434
TTGAACG27850.020.9752377
TCGTATG45050.019.244842-43
AACGTAT12400.018.38515
CCGACAT39950.018.2315771
ATCGAAG58500.016.5616846
CTCGTAT49800.016.55066742-43
ACGTGCG124300.016.5442476
CATCGAA45000.016.4648785
AACACGT122100.016.4150893
TAACACG124700.016.1169972
ACGTATT13700.015.9465786
CGCGAGT55750.015.92939910-11
CGACATC44700.015.8375852
CGTATGC52750.015.71537844-45
GCGAACG7150.015.2773767
TATGCCG54900.015.14319646-47
CGTATTC14600.014.9634577
Produced by FastQC (version 0.11.5)