FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13394325
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC160330.11969994755241492No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT156560.11688532270196521No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG136140.10164006024939666Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA50950.030.4709959
GTGCGCG67500.023.7033218
GAACGTA10500.022.1669314
AACGTAT10850.021.8841025
TTGAACG26000.021.1820377
CGTATCA39300.020.2467448-49
AGAGCGT81300.019.27118
CCGACAT38750.018.1534751
ACGTATT12650.017.6414456
ACGTGCG115700.017.1952116
AACACGT116050.016.94583
TAACACG118750.016.4817962
CGCGAGT52800.016.05243310-11
AAGAGCG99450.015.9927497
ATTGAAC46200.015.3133836
CGACCTA6550.015.2291894
ATCGAAG56000.015.0076296
CGACATC45000.014.9905232
ACACGTG134250.014.8958934
CTAACAC143000.014.7243951
Produced by FastQC (version 0.11.5)