FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5977496
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT6150.030.1803051
TGCGCGA20250.027.9112749
ACCGTTG6300.025.6328053
GTATCGA11200.025.4443244
GTGCGCG26900.021.8941028
GCGAACG4200.021.486327
CCGTCAT12950.020.2128261
CCCGTAT14600.018.9064181
CTATAAC7350.018.7778031
CAGACGT16900.018.5862311
CCGACAT14100.018.5642621
TCGAAGG30250.017.8993477
ATCGAAG30950.017.1875936
AGACGTG18450.016.7330342
TACTCTA14350.016.5491569
CGAGCGA6350.016.4553364
CGCGTAG2053.2861545E-416.2509331
TGGCGAC22000.016.2245521
CGACCTA2355.1438063E-516.1688794
CGTATCG20550.015.7164643
Produced by FastQC (version 0.11.5)