FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13144423
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA24400.029.3891169
TAACACG43450.022.5273362
ACGTGCG43950.022.2608856
GTGCGCG36100.020.1272148
AACACGT52050.019.1623693
CGCGAGT21650.016.78048910-11
ACACGTG61400.016.2443224
CGTGCGC64300.015.5109777
CTAACAC75800.015.0096931
TTGAACG25150.014.729487
CGAGTCG26800.013.99893612-13
CCCGTAT19800.013.7041711
TCGCACG28500.013.33490524-25
CACGTGC73550.013.3025585
TCGAACG12200.013.23848540-41
GTATCGA15950.012.8043764
CTCGGAT36650.012.5991381
CTCAGAT99350.012.4580061
CGATAAC14600.012.3900721
CGTATCG19850.011.96355153
Produced by FastQC (version 0.11.5)