FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13144423
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA25100.031.9672999
TAACACG44500.023.055882
ACGTGCG45100.022.8455126
GTGCGCG37000.022.7124778
AACACGT51200.019.9456523
CGCGAGT24000.018.20200210-11
CGTGCGC65150.016.3247
ACACGTG64550.015.8939654
TTGAACG24700.015.185297
TCGCACG29200.015.12586824-25
CTAACAC78250.015.0005521
CGAGTCG28950.014.84543512-13
CTCAGAT96100.013.9945091
CACGTGC75600.013.9447825
TCGAACG12850.012.38176440-41
CGCGTAA2700.002666812212.3205161
CTCGCAT41050.012.1554431
CGCACGA34700.011.97527726-27
GCGCAAT34450.011.92795844-45
CTCGGGT42800.011.76946451
Produced by FastQC (version 0.11.5)