FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7173490
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA11400.018.3321329
TAACACG15000.015.1990042
CTCAGAT52700.013.0019321
CGACCGT5956.330083E-1012.7955511
ACGTGCG19100.012.6824156
CGCCAAC18800.012.4020711
AACACGT19250.012.0901173
TAGGTCG3952.524699E-512.0245293
CGAAGAT33450.011.9492091
GTGCGCG20050.011.6077188
CTCGGGT30600.011.0406061
CGCGAGT9950.010.97918110-11
GTATCGA9205.456968E-1210.84168154
CACGCGT5303.891606E-610.7540129
CGTATCG9003.6379788E-1110.5548653
CGCATAT4155.2156445E-410.31930351
CGGGATT11750.010.124141
CTAACAC29150.010.12066651
ATCGAAG24150.010.0303996
CGCAAAT9357.6579454E-109.6693651
Produced by FastQC (version 0.11.5)