FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10732144
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTAG28500.022.831585
ACCTAGC30700.020.8860156
TGACCTA33250.020.5698474
TAGCAGT46600.014.2692889
TGTTACG24400.013.13948158-59
CTAGCAG56150.013.1111918
CGACCTA6151.0986696E-912.3567734
TACGGTC25950.012.17179460-61
CCTAGCA64000.011.3545957
CGTATCG10250.011.1210973
CTCTGAC87750.010.8994111
GTTACGG31800.010.7539958-59
CCCGTAT15750.010.5740551
CGATCGG10800.010.5547449
TCGGATT19050.010.4716362
CTCCCGT36200.010.384191
CTCGCGT9751.4551915E-1110.24871
TCTGACC75050.010.1258042
CGATTGG9902.0008883E-1110.0749834
CTCAGAT93200.010.0578331
Produced by FastQC (version 0.11.5)