FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10732144
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGC27900.022.8094566
GACCTAG29700.021.5869715
TGACCTA34900.018.234074
CGACCTA6150.016.9883754
TAGCAGT48200.014.0897529
TACGGTC26200.012.05367760-61
CTAGCAG54550.011.4919458
TGTTACG27150.011.45659258-59
GTTACGG30650.011.38782758-59
GACCGTA17400.011.05339972-73
CTCTGAC86550.010.6991051
TCGGATT20750.010.5276112
TACGCAC11050.010.314869
TCTGACC73000.010.2783722
GATTTAA58650.010.2027425
CCTAGCA61950.010.1192197
GTCCGAT9459.094947E-1110.050281
CTCGCGT10951.8189894E-129.9745581
CTCGGAT32650.09.8902131
CTCGGCT61100.09.8705751
Produced by FastQC (version 0.11.5)