FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15443201
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA30800.041.4795889
TAACACG42950.030.7407512
GTGCGCG42200.030.4992898
ACGTGCG49450.026.9881366
AACACGT55750.023.9383543
CGCGAGT32000.023.07880410-11
ATCGGAA67300.022.3971821
CGTGCGC62200.021.2269347
CGCGTTC17150.020.492769
TCGCACG37400.020.38200224-25
ACACGTG67850.019.8793134
CGAGTCG37200.019.78896112-13
CCGCGTT17650.019.1049778
CTAACAC79850.018.638831
CGACCTA9750.018.023124
TCGGAAG83050.017.8993632
ACTCCGC26800.017.189765
CACGTGC79250.016.8998435
CAATCGG21750.016.813744
TCCGCGT22450.016.7125847
Produced by FastQC (version 0.11.5)