FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15407204
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA28150.031.2146039
GTGCGCG40200.022.921878
TTGAACG37800.020.1049297
GTATCGA25000.019.5656384
TGACCTA35000.018.0459754
GACCTAG34250.017.8876845
ACGTGCG65950.017.4290986
AACACGT66400.017.0218073
CTCAGAT118900.017.011451
CCCGTAT32150.016.9836271
ATTGAAC55650.016.5581176
CGCGAGT27800.016.0604910-11
TCGAACG22300.015.97719640-41
CGAACGG23200.015.76692140-41
CGTATCG35650.015.4523753
CGACCGT12600.015.4499441
TCAGATT116700.014.893032
ACGCTAC29250.014.6130765
CGAGTCG31300.014.41336812-13
GAACGCT55450.014.3903799
Produced by FastQC (version 0.11.5)