FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10781983
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA37200.030.7642259
GTGCGCG47500.024.7930498
CGACCGT10850.022.8233721
ACCGTTG11850.020.4501113
TGGCGAC32150.017.6267471
AACACGT74900.017.5728343
GTATCGA17200.017.1280174
CGCGAGT38350.016.71630510-11
ACGTGCG82400.016.3091436
GCGAACG6500.016.0724187
TAACACG83850.015.5838052
GACGCTA30700.015.4776674
CGAGTCG42650.015.30930712-13
CCGGTAT63250.015.1335821
ACGCTAC32050.014.8257225
ACACGTG88000.014.794894
ATCGAAG48150.014.3988716
CGGTATT67600.013.9878272
CACGTGC99000.013.4869885
CAGACGT26150.013.4761621
Produced by FastQC (version 0.11.5)