FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10781983
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA36650.027.7329259
GTGCGCG47700.021.906268
CCGACAT24550.018.760531
AACACGT76500.017.8168133
TGGCGAC33150.017.1878931
ACGTGCG82650.016.9528646
CAGACGT24200.016.8736111
CGACCGT10700.016.4188561
TAACACG87700.015.5946722
ACACGTG87900.015.5060994
GTATCGA16750.015.3104694
ATCGAAG46550.015.3050776
CGCGAGT36750.015.1865310-11
CCGGTAT61300.015.1042471
CGACATC28700.015.0571522
GAACGTA6350.014.9577334
CGGTATT64650.014.6173252
AACGTAT8450.014.6133015
GACGCTA28400.014.5482394
ACCGTTG11150.014.4815023