FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16035923
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA40200.024.928849
TAACACG72950.016.9916322
GTGCGCG66300.016.2614028
ACGTGCG84450.015.8034656
CGCGAGT34900.015.65013310-11
AACACGT82200.015.3684393
CGACCTA8200.015.0584214
CCCGTAT27650.014.256171
CGTATCG29950.013.9542273
ATCGAAG61550.013.8124476
CTAACAC101200.013.7970671
GTATCGA25350.013.6761814
ACACGTG98150.012.9677444
CGAGTCG43150.012.877707512-13
CTCGGAT56600.012.4183871
CGTGCGC114450.011.6610117
CCGGTAT86100.011.5834011
TCGCACG48900.011.50917824-25
CGGTATT81850.011.4885822
CGAAGAT52500.011.4885261
Produced by FastQC (version 0.11.5)