FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16512382
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAACG29850.014.001417
CTTTAGC75950.013.524421
CGTATCG16800.011.8733543
TGCGCGA22700.011.2979939
CCCGTAT25100.011.1781761
TCGAACG15550.010.84356640-41
TTTAGCC89600.010.8132332
GACCTAG46250.010.7822895
TTAGCCT95350.010.0615313
GTATCGA18050.09.7354934
ACCTAGC46950.09.7111146
AACGTAT16150.09.7045375
CTCGGAT55450.09.6910211
CCGTATC31150.09.6054112
ATTGAAC66650.09.3347896
CTCAGAT163200.09.2370181
CTCGAAT46450.09.2140261
CGGGAAC64600.09.128131
GAACGCT43400.09.0828429
CATCGTT55800.09.0220479
Produced by FastQC (version 0.11.5)