FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17600614
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG304220.17284624275039495Illumina Single End PCR Primer 1 (100% over 50bp)
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT195130.11086545048939771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA63550.031.4641069
TTGAACG32800.026.3535427
GTGCGCG81350.024.6374848
CGACCGT15450.024.0004751
ACCGTTG14950.021.919263
GTATCGA24100.021.2827224
GAACGTA12600.018.4691124
ACGTGCG131300.018.4112036
GCCGTAT109500.018.3938346-47
CTCAGAT169550.018.3652671
AACACGT139150.017.406243
TCAGATT175700.016.6504882
GAACGCT53550.016.4082079
TCGAACG24250.016.15955240-41
CGCGAGT68100.016.00608310-11
ATTGAAC61150.015.9992586
AACGTAT14350.015.8856915
CGTATCA131550.015.5808148-49
CCCGTAT33150.015.487951
CTCGGTG142250.015.44331136-37
Produced by FastQC (version 0.11.5)